She was born in İstanbul on 5th July, 1982. During her university education, she played in some commercials. Tomris Giritlioğlu had discovered Tuba Büyüküstün and also Beren Saat for working as a actress, that was the name very effective for them. Tuba Büyüküstün is known firstly in tv series Çemberimde Gül Oya directed by Çağan Irmak. Later, she had played in Ihlamurlar Altında with ex-boyfriend Bülent İnal. In 2007, Tuba Büyüküstün had shared the leading role with Murat Yıldırım in Asi. When talked about her cinema career, she played in Babam ve Oğlum and in the other movie Sınav which she had the opportunity to work Jean-Claude Van Damme. Her first leading role was in movie Yüreğine Sor which tells about the love between muslim girl and christian boy. In 2010, she acted gipsy girl in Gönülçelen which she played with Cansel Elçin and Onur Saylak. In 2011, she married with Onur Saylak in Paris, they have twin girls Toprak and Maya. In 2013, she played with actor İlker Aksum in tv series called 20 Dakika. Now, Tuba Büyüküstün is leading actress in one of the most watched tv series Kara Para Aşk, her partner is Engin Akyürek who is winner of "Türkiye’nin Yıldızları" (Turkish Stars) where Beren Saat became second.